Description

BioMarq Tris-EDTA Buffer (pH 9.0)  is used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

BioMarq Tris-EDTA Buffer (pH 9.0) works excellent for many antibodies but may give background in some tissues (due to revealing of endogenous biotin after this pre-treatment). Tris-EDTA buffer is very useful for low affinity antibodies or when tissue antigens are not intense.

In immunohistochemistry (IHC), most commonly used fixatives such as formalin, mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking of antigens results in poor or no staining in IHC. The use of BioMarq Tris-EDTA buffer, pH 9.0 on FFPE tissue sections improves accessibility of antibodies to tissue antigens.